esxcli network ip route ipv4 add --gateway 192.168.0.1 --network 192.168.100.0/24      # add satic route
esxcli network ip route ipv4 list                                                      # list routes
esxcli network ip route ipv4 remove -n 192.168.100.0/24 -g 192.168.0.1                 # remove route

For persistent routes add commands to /etc/rc.local file